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maxpar cas  (fluidigm)


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    Structured Review

    fluidigm maxpar cas
    Maxpar Cas, supplied by fluidigm, used in various techniques. Bioz Stars score: 94/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/maxpar+cell+acquisition+solution+cas/Maxpar+Cell+Acquisition+Solution/med_rxiv__64898__2026__03__17__26348492-61-14-18
    Average 94 stars, based on 19 article reviews
    maxpar cas - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Labeling:

    Article Title: Mass cytometric single cell immune profiles of peripheral blood from acute myeloid leukemia patients in complete remission with measurable residual disease.
    Article Snippet: .. To allow for distinction between single cells, cell aggregates, and debris, DNA was labeled with iridium-191/193 by incubation in 0.1 nM Ir-nucleic acid intercalator (Standard BioTools) diluted in a buffer containing MaxPar phosphate buffered saline (PBS) (Standard BioTools) and 4% paraformaldehyde (PFA) (Alfa Aesar, 16% PFA, methanol-free) overnight at 4 C. Immediately before sample acquisition, cells were washed first in MaxPar Cell Staining Buffer, next in MaxPar Cell Acquisition Solution (CAS) (both from Standard BioTools) and then left pelleted until analysis on the mass cytometer. .. The cells were resuspended in CAS supplemented with a 1:10 dilution of the EQ Four Element calibration beads (Standard BioTools).

    Incubation:

    Article Title: Mass cytometric single cell immune profiles of peripheral blood from acute myeloid leukemia patients in complete remission with measurable residual disease.
    Article Snippet: .. To allow for distinction between single cells, cell aggregates, and debris, DNA was labeled with iridium-191/193 by incubation in 0.1 nM Ir-nucleic acid intercalator (Standard BioTools) diluted in a buffer containing MaxPar phosphate buffered saline (PBS) (Standard BioTools) and 4% paraformaldehyde (PFA) (Alfa Aesar, 16% PFA, methanol-free) overnight at 4 C. Immediately before sample acquisition, cells were washed first in MaxPar Cell Staining Buffer, next in MaxPar Cell Acquisition Solution (CAS) (both from Standard BioTools) and then left pelleted until analysis on the mass cytometer. .. The cells were resuspended in CAS supplemented with a 1:10 dilution of the EQ Four Element calibration beads (Standard BioTools).

    Article Title: A Phase II Clinical Trial of Nivolumab and Temozolomide for Neuroendocrine Neoplasms
    Article Snippet: Next, PBMCs were resuspended in 125 nmol/L Cell-ID Intercalator-Ir (Fluidigm). .. Following an overnight incubation at 4°C, fixed PBMCs were washed twice with CSB and twice with Maxpar Cell Acquisition Solution (CAS) to remove excess intercalator and resuspended at a final concentration of 1 × 10 6 cells/mL in CAS containing 0.1X EQ Four Element Calibration beads (Fluidigm). ..

    Saline:

    Article Title: Mass cytometric single cell immune profiles of peripheral blood from acute myeloid leukemia patients in complete remission with measurable residual disease.
    Article Snippet: .. To allow for distinction between single cells, cell aggregates, and debris, DNA was labeled with iridium-191/193 by incubation in 0.1 nM Ir-nucleic acid intercalator (Standard BioTools) diluted in a buffer containing MaxPar phosphate buffered saline (PBS) (Standard BioTools) and 4% paraformaldehyde (PFA) (Alfa Aesar, 16% PFA, methanol-free) overnight at 4 C. Immediately before sample acquisition, cells were washed first in MaxPar Cell Staining Buffer, next in MaxPar Cell Acquisition Solution (CAS) (both from Standard BioTools) and then left pelleted until analysis on the mass cytometer. .. The cells were resuspended in CAS supplemented with a 1:10 dilution of the EQ Four Element calibration beads (Standard BioTools).

    Staining:

    Article Title: Mass cytometric single cell immune profiles of peripheral blood from acute myeloid leukemia patients in complete remission with measurable residual disease.
    Article Snippet: .. To allow for distinction between single cells, cell aggregates, and debris, DNA was labeled with iridium-191/193 by incubation in 0.1 nM Ir-nucleic acid intercalator (Standard BioTools) diluted in a buffer containing MaxPar phosphate buffered saline (PBS) (Standard BioTools) and 4% paraformaldehyde (PFA) (Alfa Aesar, 16% PFA, methanol-free) overnight at 4 C. Immediately before sample acquisition, cells were washed first in MaxPar Cell Staining Buffer, next in MaxPar Cell Acquisition Solution (CAS) (both from Standard BioTools) and then left pelleted until analysis on the mass cytometer. .. The cells were resuspended in CAS supplemented with a 1:10 dilution of the EQ Four Element calibration beads (Standard BioTools).

    Article Title: Plasma testosterone concentration is correlated with circulating immune cell abundance in transgender young people on gender-affirming hormone treatment
    Article Snippet: .. Samples were washed twice in MaxPar Cell Staining Buffer (Fluidigm, USA) and resuspended in Maxpar Cell Acquisition Solution (CAS) (Fluidigm, USA) and counted. ..

    Cytometry:

    Article Title: Mass cytometric single cell immune profiles of peripheral blood from acute myeloid leukemia patients in complete remission with measurable residual disease.
    Article Snippet: .. To allow for distinction between single cells, cell aggregates, and debris, DNA was labeled with iridium-191/193 by incubation in 0.1 nM Ir-nucleic acid intercalator (Standard BioTools) diluted in a buffer containing MaxPar phosphate buffered saline (PBS) (Standard BioTools) and 4% paraformaldehyde (PFA) (Alfa Aesar, 16% PFA, methanol-free) overnight at 4 C. Immediately before sample acquisition, cells were washed first in MaxPar Cell Staining Buffer, next in MaxPar Cell Acquisition Solution (CAS) (both from Standard BioTools) and then left pelleted until analysis on the mass cytometer. .. The cells were resuspended in CAS supplemented with a 1:10 dilution of the EQ Four Element calibration beads (Standard BioTools).

    Cell Counting:

    Article Title: Uncovering Sex-Related Differences in Skin Macrophage Polarization During Wound Healing in Diabetic Mice
    Article Snippet: .. On the day of acquisition, the cells were resuspended in Maxpar® Cell Acquisition Solution (CAS) (Standard BioTools, South San Francisco, CA, USA) for cell counting, then in a solution of EqTM Four Element Calibration Beads (Standard BioTools, South San Francisco, CA, USA) and diluted 1:10 in CAS. .. Samples were processed in a HeliosTM Mass Cytometer (Standard BioTools, South San Francisco, CA, USA), where 150,000–500,000 events were captured per sample and converted to flow cytometry standard (FCS) files.

    Centrifugation:

    Article Title: Using mass cytometry for the analysis of samples of the human airways.
    Article Snippet: .. Immediately before sample acquisition on the Helios® instrument, cells were washed twice with CSB and twice with Maxpar Cell Acquisition Solution (CAS) (Standard BioTools), by centrifugation at 800 x g for 7 min at RT. ..

    Concentration Assay:

    Article Title: A Phase II Clinical Trial of Nivolumab and Temozolomide for Neuroendocrine Neoplasms
    Article Snippet: Next, PBMCs were resuspended in 125 nmol/L Cell-ID Intercalator-Ir (Fluidigm). .. Following an overnight incubation at 4°C, fixed PBMCs were washed twice with CSB and twice with Maxpar Cell Acquisition Solution (CAS) to remove excess intercalator and resuspended at a final concentration of 1 × 10 6 cells/mL in CAS containing 0.1X EQ Four Element Calibration beads (Fluidigm). ..



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    Image Search Results


    Journal: Cell Reports Medicine

    Article Title: Multi-modal analysis reveals tumor and immune features distinguishing EBV-positive and EBV-negative post-transplant lymphoproliferative disorders

    doi: 10.1016/j.xcrm.2024.101851

    Figure Lengend Snippet:

    Article Snippet: Prior to acquisition, cells were washed in Maxpar Cell Acquisition Solution (CAS; Fluidigm, # 201240), resuspended in a 10% solution of EQ Four Element Calibration Beads (Fluidigm, # 201078) in CAS, and filtered using polystyrene FACS tubes with cell strainer caps (Fisher Scientific, # 08-771-23).

    Techniques: Recombinant, cDNA Synthesis, SYBR Green Assay, Blocking Assay, Cell Stimulation, Staining, Electron Microscopy, Conjugation Assay, Antibody Labeling, Gene Knockout, DNA Extraction, Enzyme-linked Immunosorbent Assay, CyQUANT Assay, Proliferation Assay, Gene Expression, Microarray, Software